Analysis Note

ControlStimulated Jurkat Cells

Application

Research CategorySignaling

Research Sub CategoryCell Cycle, DNA Replication & Repair

Detect Ki67 using this Milli-Mark Anti-Ki67 Antibody, clone Ki-S5 APC conjugate validated for use in FC, IC, IH, FC, IH(P).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a marker of cell cycling and proliferation. This specificity of Ki-S5 antibody for proliferating cells might make it a useful tool for determination of the proliferative fraction in tumors such as Non-Hodgkin′s lymphoma (Kreipe et al., 1993), mammary carcinomas (Mauri et al., 1984), ovarian tumors (Ballin et al., 1994) and prostate cancer.

Immunogen

Nuclear protein preparation from the human cell line U937.50

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Protein A purified

Purified mouse monoclonal IgG1 conjugated to Allophycocyanin (APC) in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Quality

Evaluated by Flow Cytometry with Jurkat cells.

Specificity

Antibody recognizes Ki67.

Storage and Stability

Maintain refrigerated at 2-8oC in undiluted aliquots for up to 6 months from date of receipt. Protect from light.

Target description

345 & 395 kDa observed